Separation and Identification of Plant Colors by Paper Chromatography
All living organisms require energy for his or her chemical processes. The ultimate method to obtain this energy is the sunshine. Plants convert light energy into the substance energy of sugars. During photosynthesis pigments are used to get light strength. Pigments of green plants can easily be separated and recognized using a technique called newspaper chromatography. The purpose of this test is to distinct the colors using conventional paper chromatography. In this process, different pigments can separate over the chromatography conventional paper depending on polarity of the colors. В One of the most polar will probably be in the most affordable band within the paper, as the least polar will be in the uppermost music group. В В Using conventional paper chromatography, the pigments that give a flower its color can be segregated and observed to determine the value of each color and their function during photosynthesis.
This materials had been obtained to conduct this kind of experiment: a capillary conduit, beakers, extractions of leaf pigments in acetone, forceps, scissors, acetone, a quart jar which has a lid that contains solvent of petroleum azure and acetone and chromatography paper stapled into a tube marked with a pencil collection about 1cm from one end. An draw out of chloroplast pigments wherever obtained and a mixer was used to rupture the cells. Pigments were after that extracted with acetone an organic solvent. Then simply pigment components were separated using newspaper chromatography. Color extract was applied to a cylinder of chromatographic daily news then the cylinder was put in a container with the organic solvents.
Paper chromatography proved to be an exact method of distancing and noticing the various shades of herb pigments. The pigments dissolved in the solvent and...